Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries-inducing Streptococcus mutans.

J Cell Mol Med. 2018 Jan 8. doi: 10.1111/jcmm.13496. [Epub ahead of print]


Streptococcus mutans contributes significantly to dental caries, which arises from homoeostasic imbalance between host and microbiota. We hypothesized that Lactobacillus sp. inhibits growth, biofilm formation and gene expression of Streptococcus mutans. Antibacterial (agar diffusion method) and antibiofilm (crystal violet assay) characteristics of probiotic Lacobacillus sp. against Streptococcus mutans (ATCC 25175) were evaluated. We investigated whether Lacobacillus casei (ATCC 393), Lactobacillus reuteri (ATCC 23272), Lactobacillus plantarum (ATCC 14917) or Lactobacillus salivarius (ATCC 11741) inhibit expression of Streptococcus mutans genes involved in biofilm formation, quorum sensing or stress survival using quantitative real-time polymerase chain reaction (qPCR). Growth changes (OD600) in the presence of pH-neutralized, catalase-treated or trypsin-treated Lacobacillus sp. supernatants were assessed to identify roles of organic acids, peroxides and bacteriocin. Susceptibility testing indicated antibacterial (pH-dependent) and antibiofilm activities of Lacobacillus sp. against Streptococcus mutans. Scanning electron microscopy revealed reduction in microcolony formation and exopolysaccharide structural changes. Of the oral normal flora, L. salivarius exhibited the highest antibiofilm and peroxide-dependent antimicrobial activities. All biofilm-forming cells treated with Lacobacillus sp. supernatants showed reduced expression of genes involved in exopolysaccharide production, acid tolerance and quorum sensing. Thus, Lacobacillus sp. can inhibit tooth decay by limiting growth and virulence properties of Streptococcus mutans.
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Five Tips for a Fabulous Floss

Dental Floss

Do you floss every day (great job!)  but still find that you are not cleaning between your teeth as well as possible? Get the most out of your dental flossing by upgrading your techniques, upgrading your floss, and spending a little more time to perfect your flossing habits. Here are 5 tips to make sure you are doing it right.

The Right Product: When deciding on a dental floss, look for a product that features the ADA Seal of Acceptance by the American Dental Association. You can also request product recommendations from your dentist. There are many options, including flavored floss and floss for sensitive gums.

The Right Length: A single thread for a flossing session should be about 18 inches long. At this length, you can clean between each tooth with a new section of the thread, which avoids spreading any debris between teeth.

The Right Method: Work gently between teeth and use steady motions. Remember: your goal is to remove debris, not grind your teeth away!

The Right Time: Try to floss for about 2 to 3 minutes per session. Make sure you work every area between and around each tooth. This includes behind the back row!

The Right Support: It can be hard to remember to floss. Write yourself a reminder, and make it fun! Maybe listen to one of your favorite songs as you floss. Regular visits to your dentist are also helpful, to check in on how well your oral health habits are working.

No matter the needs you have for your teeth, our team at Hollywood Dental Care can help. To schedule a visit to our dentist office in Hollywood, Florida, call us at (954) 367-5671. We look forward to seeing you and your radiant smile!

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